FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35513488
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC14913504.1993903837325135TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC580140.16335765160549706TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1714900.043.378621
ATCGGAA1733150.043.2752382
CGTATGC1679450.043.27497544
CGTCTGA1734550.043.13966416
ACACGTC1738550.043.11568513
TCTCGTA1607800.043.08373341
CTCGTAT1611900.043.0544842
ACGTCTG1739450.043.01542715
TCGTATG1675600.042.99736443
TCGGAAG1742750.042.9971123
GATCTCG1610700.042.9383639
CGGAAGA1747000.042.9024664
ATCTCGT1615750.042.7662140
CACGTCT1753550.042.7193114
GCACACG1755800.042.710711
CACACGT1760050.042.5952512
AGTGATC1671900.041.83848636
GTGATCT1671100.041.51067437
GTCACAC1791650.041.41256729
GAACTCC1804950.041.2868721
Produced by FastQC (version 0.11.2)