HKFN7BGX2_n01_mb23.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKFN7BGX2_n01_mb23.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36135597
Sequences flagged as poor quality	0
Sequence length	50
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	3681509	10.18803978802398	TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC	75623	0.20927563477088812	TruSeq Adapter, Index 4 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	399960	43.830025	1
ATCGGAA	404120	43.73804	2
TCGGAAG	404645	43.631935	3
CGGAAGA	408970	43.15701	4
ACACGTC	409200	43.036327	13
CGTCTGA	409160	42.94806	16
ACGTCTG	409360	42.936687	15
GCACACG	411215	42.866642	11
CACGTCT	410510	42.849277	14
CGTATGC	405700	42.816387	44
CACACGT	411530	42.805614	12
AATCTCG	395650	42.633648	39
CTCGTAT	397675	42.58283	42
TCTCGTA	396985	42.579136	41
ATCTCGT	397245	42.510147	40
TCGTATG	408345	42.502415	43
ACCAATC	396605	42.297436	36
GAAGAGC	418780	42.194553	6
GACCAAT	407085	42.179108	35
GAGCACA	418520	42.16294	9