Basic Statistics
Measure | Value |
---|---|
Filename | HKFN7BGX2_n01_mb22.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 44733681 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC | 1614602 | 3.6093653906996837 | TruSeq Adapter, Index 2 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 174500 | 0.0 | 43.13933 | 44 |
CGTCTGA | 181400 | 0.0 | 42.908077 | 16 |
ACACGTC | 181870 | 0.0 | 42.883015 | 13 |
GATCGGA | 181470 | 0.0 | 42.870926 | 1 |
CACCGAT | 179410 | 0.0 | 42.864258 | 31 |
TCTCGTA | 173905 | 0.0 | 42.856537 | 41 |
CTCGTAT | 173745 | 0.0 | 42.847748 | 42 |
ATCGGAA | 182830 | 0.0 | 42.847187 | 2 |
CGATGTA | 178730 | 0.0 | 42.84717 | 34 |
TCGTATG | 174110 | 0.0 | 42.83266 | 43 |
ACCGATG | 179325 | 0.0 | 42.81583 | 32 |
ACGTCTG | 181885 | 0.0 | 42.811707 | 15 |
CCGATGT | 179270 | 0.0 | 42.730743 | 33 |
TATCTCG | 175265 | 0.0 | 42.715584 | 39 |
GTCACCG | 180320 | 0.0 | 42.688534 | 29 |
TCGGAAG | 183825 | 0.0 | 42.581806 | 3 |
ATCTCGT | 175770 | 0.0 | 42.545345 | 40 |
TCACCGA | 181005 | 0.0 | 42.52894 | 30 |
CGGAAGA | 184240 | 0.0 | 42.480927 | 4 |
CACGTCT | 183465 | 0.0 | 42.474678 | 14 |