FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44733681
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC16146023.6093653906996837TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1745000.043.1393344
CGTCTGA1814000.042.90807716
ACACGTC1818700.042.88301513
GATCGGA1814700.042.8709261
CACCGAT1794100.042.86425831
TCTCGTA1739050.042.85653741
CTCGTAT1737450.042.84774842
ATCGGAA1828300.042.8471872
CGATGTA1787300.042.8471734
TCGTATG1741100.042.8326643
ACCGATG1793250.042.8158332
ACGTCTG1818850.042.81170715
CCGATGT1792700.042.73074333
TATCTCG1752650.042.71558439
GTCACCG1803200.042.68853429
TCGGAAG1838250.042.5818063
ATCTCGT1757700.042.54534540
TCACCGA1810050.042.5289430
CGGAAGA1842400.042.4809274
CACGTCT1834650.042.47467814