FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13053632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482630.3697285169368954No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN346080.2651216151949128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT12800.038.9027826
ACTCTAG14000.036.052865
TCTAGTT14900.033.6235167
CTATCCC7000.033.515951
TTGTACT15450.032.9369541
CTAGTTG15150.032.408378
TGTACTC16150.031.6964282
GTACTCT16800.030.0695923
TACTCTA20600.027.076994
TGGACAC17550.024.58224728
TACCTCT18050.023.7092516
TGTTACC21000.023.66923513
GGACACC18250.023.6386429
ACCTCTA18800.023.11761717
GATAGAG8350.022.8736532
TATCCCC10200.022.3408682
GTTACCT19850.021.22589514
CACAGGG8750.021.07782625
GGATAGA9050.020.37707131
TTAGAGT17850.020.28645312
Produced by FastQC (version 0.11.5)