FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798441
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA61000.12712462235130118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG9350.033.5725985
CTCTAGT10950.028.9641176
TGTGATC20750.024.4133665
ATCTCTC23050.022.14692569
TCTAGTT15300.020.9418327
AGGACTA10000.020.64968553
CTAGTTG15600.020.3295258
GTGATCT25950.020.12193566
CGCTAGC4850.020.0863366
TGTCCTC24800.019.51452621
TAGTTGT18350.019.4207469
GCTAGCA5300.019.11659667
GGCGTGA12250.018.9643291
CCTCCGC25200.018.81234724
GTCCTGG4350.018.78880336
TACAAGA26650.018.71329753
GACTATC11250.018.70229755
ACAAGAC26950.018.65025154
CCCATGT25950.018.5213930
GGACTAT10950.018.50303554
Produced by FastQC (version 0.11.5)