FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310068
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC96790.1822763851611693No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT76540.14414128029998863No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT73870.13911309610347738No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG71910.13542199459592608No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA70390.13255950771251895No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA68310.12864242039838286No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA63570.11971598103828426No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA61320.11547874716481973No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA57590.108454355010143No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT56450.10630748984758764No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA55960.1053847144706998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT8750.025.470976
ACTCTAG9100.024.8519525
TGTGATC20100.022.59051965
GTCTAAC5150.020.3783871
ATCTCTC23100.020.32870969
GTGATCT22800.020.2557666
TCTAGTT12900.019.5627217
GTGGAGA11100.018.0232831
CTCCGAC19400.017.7506661
GTGATAG16400.017.5884864
TACAAGA26300.017.5579753
CGACTGG17350.017.381134
GGCGTGA15900.017.3264351
CTAACGT22650.017.2308141
ACAAGAC26600.017.21483454
CCCATGT25100.016.97485530
AGGACTA8700.016.94913153
TGTCCTC25500.016.83790421
GATCTCT27550.016.76338868
TCCGCCC24950.016.55099926
Produced by FastQC (version 0.11.5)