FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6037105
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA91450.1514798897816089No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC72920.12078637028840811No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA68080.11276928262801458No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT64180.10630923265373055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGATC29350.026.35928765
ATCTCTC28500.026.32698669
TGTCCTC38200.021.0001821
CTAATTA8800.020.8393063
CCTCCGC38350.020.65967624
GTGATCT37950.020.38590466
TACAAGA37400.020.0588553
GATCTCT38250.020.0227468
ATACAAG38100.019.89435852
ACAAGAC37400.019.74763754
ATTGTCC40100.019.51507619
TGTTGAG41500.019.40596839
TCCGCCC41800.019.26780926
CCCATGT41500.019.24979230
TGATCTC40050.019.2197267
ATTGTAA44550.018.8147376
TTGTCCT42300.018.80973220
AGAATTG43650.018.45011716
GTCCTCC43750.017.96194822
GCCCATG45850.017.78051429
Produced by FastQC (version 0.11.5)