FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089207
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC2603.6561687E-1019.41886153
CTCCACT6400.017.60104667
TCCCCTA4450.016.58494664
ACTACTG8150.016.4801625
TCGGTGG3304.9112714E-1016.4766151
GGGGACC1403.0888093E-416.3831212
CTCCGAC7750.015.6495771
CGGTGGT3351.0602889E-815.07135652
GCTAGAT2001.8549561E-514.7507831
GATTCGA13150.014.6988224
CACTACA1857.0492475E-414.69761264
GGGGAAT13850.014.6738621
CTACCTG7850.014.60607819
GTGGCAC5300.014.436172542
CTAGATT2703.977395E-614.38544757
ACCCTAC2554.268226E-614.28790242
GGTGGTG7150.014.2017427
CTAGTTG3254.3964974E-914.1101188
TAGATGG8400.014.03880931
CGACTGG8200.013.9833274
Produced by FastQC (version 0.11.5)