FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777830
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT7300.020.2094737
ACTCTAG6950.019.3413645
CTCTAGT7550.018.2381736
GGAGTGT9500.017.9450668
CTAGTTG8300.016.9846158
GATTCGA19400.015.7181384
ACTAGAT6650.015.16270652
GTACTCT9600.014.3467183
ACGGTGG16800.013.8556866
GGATTCG24800.012.9583243
CTAGATG8100.012.9271853
AGGAGTG15000.012.67657857
AGTACTA8600.012.62607849
ATGCTAC7700.012.59343669
TCGACAG24000.012.5672977
GTCTAAC3401.1785414E-712.5446131
CCTACTA7650.012.4289334
ACGTAGG4753.8198777E-1112.424417534
GGGGAAT22200.012.4142121
CCTCGTA14150.012.33335153
Produced by FastQC (version 0.11.5)