FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6373495
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA83420.13088580127543836No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT68900.10810395238405301No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT65890.1033812688328774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG7750.020.2729665
GTCTAAC7500.019.6533241
CTCTAGT8300.018.9289846
GTAGGGA7350.018.25960419
GGTGGTC7850.017.83597653
TGTAGGG8300.017.74717518
CTAATTA10050.017.5905953
GTCGTGT7700.017.42551814
GGGGAAT26900.016.6821881
GATTCGA39250.016.5961844
AGGACTA7650.016.26868853
CTAGATT8250.016.02895457
GTGATAG12750.015.9175544
TCTAGTT11650.015.7325457
GGATTCG41700.015.5446853
TGCTACG13400.015.09451970
GGGTAAT13150.014.9454951
CTAGTTG11450.014.5781218
ATTCGAC45700.014.3965245
AGTGTGC8650.014.37875829
Produced by FastQC (version 0.11.5)