FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979049
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2681303.360425534421458No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG2672593.3495094465518385No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA944041.1831485180752743No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA711960.8922867875607732No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT657350.8238450471979806No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT348160.4363427270593275No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG315510.3954230635756216No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC295840.37077100291024656Illumina Single End PCR Primer 1 (96% over 33bp)
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG288660.3617724367903995No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA265410.3326336258869948No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG256120.3209906343475269No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA230910.28939539035291045No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG226400.2837430876787447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203670.2552559835138248No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA198600.24890184281359845No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT193130.24204638923761462No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA192260.2409560337328421No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA162780.20400927478951436No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC147650.18504711526398698No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA135920.17034611518239828No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG134820.1689675047740652No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT128830.16146034445959662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTTTTTTTTTTTTTTTTT125010.1566728064961125No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC123560.15485554732149157No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT120570.15110823357520425No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTGTACCCTACAG118170.14810035632065927No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA114600.14362613890452358No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG113800.14262351315300859No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG111900.14024227699316044No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT111370.13957803743278177No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG109800.1376103843954336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102930.12900033575429853No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA102400.12833609619391986No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT101630.12737106890808667No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG99780.12505249685770822No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT91670.11488837830172492No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTATCCTACAG89200.11179277129392236No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA81450.10207983432612082No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG80970.10147825887521181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA44200.064.0369162
CCGTATC44150.063.31596461
GCCGTAT47500.063.0423960
GTATCAT46750.059.37486663
CGCCGTA49200.058.87937559
TGGTCGC50150.058.77516655
TCGCCGT50150.057.8418658
GTGGTCG51750.057.41018754
TCGGTGG49950.056.50813351
TTTTTTC536300.055.690941
TCTCGGT44550.055.55797649
CGGTGGT53050.054.60427552
CTCGGTG46500.053.98413550
GGTCGCC54750.053.98040856
TAGATCT46550.053.83946245
GGTGGTC56050.053.14434453
GTCGCCG57150.051.16700457
AGATCTC46800.050.88298446
TGTAGAT57150.050.1385443
TTGTACT629900.049.9785421
Produced by FastQC (version 0.11.5)