FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284881
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG8900.033.8528185
CTCTAGT11900.025.0430566
TCTAGTT12300.024.7602067
CTAGTTG11850.024.594848
GTACTCT12500.023.845733
TACTCTA16500.021.6349544
TACCTCT15100.018.86626216
CTTAGAG10900.018.02419911
GACACCT17000.017.72277630
CTAGATT10050.017.3999557
ACCTCTA16450.017.3205917
CCTACTA13200.017.1184334
CCTTAGA11850.016.8558610
ACTATCA12150.016.63139256
TACTATC13750.016.19353136
TAGTTGT19100.016.1163549
TGGACAC18050.015.96608328
ACCCTAC11600.015.69772342
TGTACTC19600.015.5431492
ACCTTAG12900.015.2311929
Produced by FastQC (version 0.11.5)