FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spoVG_T0_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spoVG_T0_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033194
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA63060.1563525087064991No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT51040.1265498262667256No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC45560.11296258002962416No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA43970.10902029508126809No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG42890.10634251662578097No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT42190.10460691947870597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT7000.036.45820221
CAACTAG7800.031.46048418
ACTAGAG8400.030.3818420
AACTAGA8500.028.86961719
CCAATAG8850.027.35512538
CAATAGA10350.022.75843239
GTACAAT11150.022.59371426
TCGCTTA9250.022.29939352
AGAGGTA12850.021.38531510
ATTAGAG12650.021.2075127
CTATAGT3450.020.86189837
TAGAATC11800.020.69852642
TAGAGGT13300.020.663039
ACAACTA12350.019.86977817
CTAATTA4400.019.3379753
GAGTACA13450.018.97335424
ATAGAAT13250.018.7647441
GGGATAG8850.018.1321961
TATAGTG4450.017.64417838
GGGTAAT8800.017.4909231
Produced by FastQC (version 0.11.5)