FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332840
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG33680.14437338180072357No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC32390.1388436412270023No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA25570.1096088887364757No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT25510.10935169150048868No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT23470.10060698547692941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG9600.041.253825
GTACTCT10200.038.510373
CTCTAGT10300.038.447696
TACTCTA11550.037.975264
TTGTACT11700.034.1510051
TGTACTC12300.032.7380262
TCTAGTT13950.029.3263427
ACCTCTA14050.027.02288217
GGTGGTC2450.026.98881553
TAGTTGT15100.026.442659
CTAGTTG15600.025.8049228
TACCTCT15000.025.08946416
GATTAGT3400.025.0274439
CTCTAAT15400.024.86652819
TAGAGTA15400.024.22528613
CTTAGAG15500.024.06951111
ACACCTA10750.024.05141831
ACCTACT11050.023.99080533
AGAGTAC15600.023.91470714
TTAGAGT16100.023.17251212
Produced by FastQC (version 0.11.5)