FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943715
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT10850.032.8663186
ACTCTAG12050.030.1370855
GTACTCT12100.028.936163
TCTAGTT14750.026.1714487
TACTCTA15400.025.2821274
TAGTTGT15400.024.6416349
CTAGTTG15500.024.4826568
TGTACTC15200.023.4682392
TTGTACT16150.022.0958881
ACCTCTA16200.021.0031117
GCCCTAG1751.0113035E-620.02053370
CTTAGAG17500.019.62634311
AGGACTA13300.019.31601553
TACCTCT17950.019.13431716
TAGAGTA17550.019.0107913
CACCTTA18550.018.8700088
TTAGAGT18200.018.51202812
AGAGTAC18250.018.46131114
GGACTAT13900.018.20302254
ACACCTA13000.018.11892531
Produced by FastQC (version 0.11.5)