FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556072
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG89980.1619489452260518No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT59760.10755800140818908No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC56720.10208651003802686No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT56350.10142057194363213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC21750.036.8778082
GTACTCT22400.036.0951233
CTCTAGT22250.035.597096
TACTCTA24650.034.6570784
ACTCTAG23800.033.555045
TTGTACT24950.032.2902571
TCTAGTT27500.029.2765527
TAGAGTA36250.028.97820513
CTTAGAG36900.028.5569511
AGAGTAC37350.028.0384114
TTAGAGT38850.027.12358912
CTCTATG6650.027.06595636
GAGTACA40000.026.01723515
CACCTTA41400.025.9293128
TTCCACC41950.025.9030555
CCTTAGA41800.025.60127410
TAGTTGT30550.025.4966569
TTTCCAC43650.025.1205334
TCCACCT43350.024.9901076
AGTACAA42900.024.48734916
Produced by FastQC (version 0.11.5)