FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787673
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA40120.1439193190879992No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT32840.11780434792746494No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC30150.10815472259479503No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA28540.10237929628044609No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG28100.1008009189026116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT9850.031.5568686
GTACTCT10350.030.350693
ACTCTAG10800.028.7810345
TAGTGTA2950.026.61446840
TTGTACT11950.026.3035261
GGTGGTC4050.025.94691353
CGGTGGT3800.025.60550552
AGATTAG3200.025.55797436
TGTACTC12300.025.5431232
TCTAGTT12950.024.5058357
TACTCTA14100.024.3660144
GGTCGCC4950.022.8037856
TCGGTGG4650.022.59892351
CTAGTTG14400.022.2650348
CTTAGAG10300.021.91494811
ACCTCTA14100.021.81181717
TAGATCT4300.021.72067845
TAGTTGT15050.021.3034219
TGGTCGC5750.020.30757955
GTAGATC4600.020.30411144
Produced by FastQC (version 0.11.5)