FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530616
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT7350.031.641663
CTCTAGT7900.030.2626696
TACTATC6900.027.5253536
TGTACTC8700.027.1118452
GGACTAT6650.026.29334654
TCTAGTT10200.025.3640867
ACTCTAG9400.024.7375345
AGGACTA7300.024.48410853
ACACCTA8550.022.98145131
CCTACTA8350.022.35506234
TACTCTA12200.021.4768414
TAGTTGT11500.021.3577619
CTACTAT9350.020.66304835
TTGTACT11900.020.6544511
CTGTAGG9400.020.2540549
ACCTCTA11500.019.93501317
TACCTCT11500.019.64762116
TAGGACT9250.019.32259452
CTAGTTG13000.018.8934068
CCTAACT6000.018.0180782
Produced by FastQC (version 0.11.5)