FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3896452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA56410.14477273170566454No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC45970.11797912562505582No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT42750.10971519731283744No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG39530.10145126900061902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAG7150.040.7426638
ACTAGAG8450.036.41834620
CAATAGA8300.035.49194739
CTAGAGT9300.033.08978721
AACTAGA9700.031.05025319
ATTAGAG10600.029.6473967
CAACTAG10550.028.85888718
TAGAATC10200.028.23151842
AGAGTAC11000.027.9759123
TAGAGTA11000.027.08306122
AGAGGTA12350.026.76927210
ACAACTA11450.026.590517
TAGAGGT12900.026.1374459
GAGTACA11650.026.13099124
GTACAAT11950.025.74890726
CATTAGA12750.025.1615336
ATAGAAT14650.021.22490741
AGTACAA14550.020.92275425
GAGGTAA15650.020.91494211
TCGCTTA12550.020.44824852
Produced by FastQC (version 0.11.5)