FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447267
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG16660.11511352086380744No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA15670.10827304153276486No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT15220.10516373274592733No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT14790.10219261546072701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGTC2750.036.64382653
CGGTGGT3050.033.03951352
GTCGTGT3750.032.35655214
GTACTCT5100.032.156343
GTGTAGA3800.031.59364742
ACTCTAG5150.031.2061825
TAGATCT3400.030.77832845
TGTACTC5450.030.696262
GTAGATC3700.028.28394144
AGTGTCT3900.027.75147629
TGTGTAG4250.027.00572641
TTGTACT6100.026.3635161
CCTAGGT500.00986795826.2559091
CTCTAGT6000.026.2377596
GGTCGCC4000.026.16478356
CTAACGT2200.025.3608231
GTAGGGA4400.025.34416819
GTGTCTT4300.025.16994530
GCGTCGT4600.024.9500812
GTGGTCG4050.024.88465954
Produced by FastQC (version 0.11.5)