FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5070954
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG9050.034.002315
GTACTCT11800.026.0821593
CTCTAGT12150.025.3258256
TCTAGTT13600.023.3465887
TTGTACT13700.023.1942521
TGTACTC15000.021.1761382
CTAGTTG14900.020.8702628
TAGTTGT14600.020.4022989
ACCTCTA15750.019.74568617
TACTCTA20850.018.99994
TACCTCT16400.018.76143316
ACACCTA12000.017.73091931
GGACACC16300.017.47152329
CTTAGAG14250.017.45727311
TACTATC12650.016.04275336
CTAACCT12800.015.9437742
CTCTAAT19700.015.78652419
GATTCGA17950.015.320994
AGAGTAC16050.014.88777614
CCTACTA14050.014.6778834
Produced by FastQC (version 0.11.5)