FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_spo0A_T0_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_spo0A_T0_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261949
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC95830.29378141718340783No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG50340.15432491433802306No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA43020.13188434276562877No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA39950.12247279157338144No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA38840.11906991801527247No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA33400.10239277192868436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGAG18700.046.78636620
CCAATAG18650.046.20049338
CTAGAGT18800.046.18890421
CAATAGA19250.044.59028639
AACTAGA20700.041.4744519
AGAGTAC20900.041.23434423
TCGCTTA16800.041.13064652
AGAGGTA22300.040.98853310
CATTAGA22500.040.1904536
TAGAGTA21550.039.9906222
TAGAATC21350.039.74781442
GAGGTAA23200.039.11603511
GTACAAT22900.038.91733626
AGGTAAC23850.038.8741712
CAACTAG22750.037.8812218
ATTAGAG24000.037.8133287
GAGTACA22950.037.40488424
ACAACTA24050.037.05983417
TCCAATA23750.036.96927337
TAGAGGT24700.036.8737689