FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19075011
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT32750.024.0919976
ACTCTAG33500.023.1622545
TCTAGTT43000.019.3384177
CTAGTTG42700.018.3240368
ACCTCTA47400.016.37024917
GTGGTGA40400.016.206898
TGTACTC50200.016.0486532
AGGTGGT38250.015.9202016
GGTGGTG39650.015.5228097
TACTATC39000.015.27725536
GTGATAG56300.015.238493
AGGACTA36550.014.99674353
TAGTTGT55900.014.8169459
TACCTCT54050.014.77846416
GATTCGA85000.013.9444064
GAGTCGA99650.013.6686734
TGGGAGT100200.013.5364231
ACTATCT43700.013.40942437
GACACCT56050.013.31767930
TGGACAC57600.013.30033828
Produced by FastQC (version 0.11.5)