FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4661754
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT11200.032.7238856
ACTCTAG11200.032.141255
TCTAGTT13200.028.7561197
CTAGTTG13900.026.365758
TGTACTC14300.026.0940132
ACCTCTA16050.021.81639117
TAGTTGT17350.021.5001899
AGGACTA12000.021.07547853
TACCTCT17550.020.32227316
TTGTACT18900.019.9235461
GACACCT18250.019.00552630
GGACTAT12900.019.00234654
CTCTAAT18550.018.87617719
GGACACC18100.018.43989829
TGGACAC18350.018.36699328
CACCTTA15000.018.3241988
TGTTACC19400.018.04641513
GACTATC13800.017.76306355
GATTCGA21600.017.5754474
TTAGAGT15500.017.30994612
Produced by FastQC (version 0.11.5)