FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784012
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA4900.025.38636619
CTCTAGT6150.022.3541646
TCTAGTT6600.021.8210587
GGTGGTC6600.020.03180753
GAGTGTG7000.019.6397328
AGTGTGC6350.019.58818229
GTCGTGT7350.019.14833514
GTGTAGA7050.019.12701242
TGTACTC7900.018.2362792
GATTCGA14650.017.8769784
ACTCTAG7650.017.5447395
TAGGGTT5850.017.34677922
CTAGTTG8200.017.1641258
TGTAGGG7450.017.13646918
CGATTAG4100.016.764968
TCGTGTA8100.016.56718415
GTGATAG10100.016.5306724
ACCTCTA8450.016.27067617
AGGACTA6100.015.93658753
AGGGGTC3952.3646862E-1115.750751548
Produced by FastQC (version 0.11.5)