FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888306
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT5050.018.1482776
AGGTGGT5550.017.6928226
TCTAGTT5900.016.6420947
TGAACTA8950.016.4607092
AGGGAGG10400.016.3635914
GGAGTCC1703.6768778E-416.01233745
GAACTAC8650.015.8950593
CAGTAGG4900.015.87424649
ACTACTG9100.015.8265985
GGACCTA1754.581957E-415.555843
GTGATAG7150.015.1090393
CTAGTTG5850.015.1058998
TGGGAGT15250.015.0348121
CTAGTGG4751.8189894E-1214.73980369
AACTACT10000.014.7300384
AGTAGGT5350.014.53932950
GTGGAGT5500.014.14367457
ACTCTAG6250.014.14034755
GGTGGTG6950.014.127827
GAGTCGA16000.014.1162874
Produced by FastQC (version 0.11.5)