FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726352
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGT7200.029.1147566
ACTCTAG7500.027.9509165
TCTAGTT8100.026.6881687
CTAGTTG9200.022.7851568
TGTACTC11050.020.1623252
TACTATC8150.019.28868936
CTATAGG1058.5528963E-418.71461911
TGGACAC11800.017.76541328
GACACCT11450.017.7363230
GCCCTAG1301.7605837E-417.6349310
ACTATCA7950.017.59149256
ACCTCTA12500.016.77117
GAACTAC9350.016.4668393
TGTTACC12750.016.43928713
TTGTACT13350.016.203481
TACCTCT12950.016.18561716
GGACTAT8950.016.0597654
AGGACTA8550.015.90137453
ATCTAAC9900.015.87885840
ACTATCT10350.015.82153737
Produced by FastQC (version 0.11.5)