FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5871557
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA4000.033.5607140
TATAGTG6250.020.4312838
CAACTAG8150.019.68932718
GTAGGGA7800.019.31326319
CACTAGC8150.019.07461560
TGTAGGG8600.019.0398718
CTAGCAC8500.018.74471562
CTAATTA9850.017.9561353
GTCGTGT8950.017.92602714
AGTGTAG9250.017.7001229
ACTAGCA8800.017.66533761
CTAGAGT9300.017.6067621
CCAATAG9200.017.0830138
TAGCACA9350.017.0406563
GTGATAG12700.017.0201054
GCCACTA9500.016.77277458
GTGTAGA7950.016.5016942
GGTGGTC8300.016.38650153
CTATAGT8200.016.37121837
CTAGTGG3801.2732926E-1116.36500569
Produced by FastQC (version 0.11.5)