FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688534
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG5250.034.7944151
CAACTAG8450.031.77525318
GGTGGTC6250.031.09287853
ACTAGAG8750.030.68581820
CTAGAGT9150.030.05975321
CGGTGGT6200.029.4630152
AACTAGA9800.027.3980519
CCAATAG10800.025.46484838
CAATAGA10700.025.09086439
GTAGGGA8000.024.55800819
AGGTAAC11650.023.88793212
GGTCGCC8300.023.88339256
GTAGATC7600.023.52421644
GTGTAGA8900.023.32855642
GGTAACA11950.022.7397913
TCGCTTA10300.022.64044652
GTGGTCG8800.022.5258154
TAGAGTA12150.022.36809722
TGTAGGG8950.021.95129218
AGAGTGT9000.021.46437327
Produced by FastQC (version 0.11.5)