FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4582174
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT3100.029.60900137
TAGGGTG3800.023.29204238
GTAGGGA5650.017.98821419
AGTGTAG5700.017.82886729
CTAACGT13400.017.3793011
CTAGTGG5800.016.71969469
TGTAGGG6300.016.1321118
CTAATTA4550.015.8539783
GAGTGTA6200.015.33356728
TAGGGTT8350.015.31274122
GGTCGCC6250.014.894279556
AGGGAGG17350.014.73729514
GGGTAAT10150.014.5420381
GGTGGTC6200.014.38823753
GTCGTGT7500.013.98657414
GTCTAGT7100.013.65834267
TGTCTAG7100.013.657989566
TCGGTGG6600.013.51622351
GTCCCTA5000.013.11255736
GGGTGTA6800.013.01614140
Produced by FastQC (version 0.11.5)