FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n02_PY79_T0_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n02_PY79_T0_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4814931
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT6700.019.05252521
CTAATTA5800.017.4961133
CTAACGT7950.016.8890481
AGGGAGG17650.016.68790814
CGGGGAG7900.016.5814061
CAACTAG6800.016.365818
CACTAGC4203.6379788E-1215.74448260
CTAGCAC5350.015.26748362
TAGGGTT8250.015.07621822
GGGGGTC2202.8031536E-614.8793933
GTGCAAC18250.014.70578463
GGTAACA8800.014.50288713
GGGAGGA21650.013.90701815
CTAGTGG5501.8189894E-1213.43836669
TACTATC5400.013.33301836
CCAATAG8600.013.31892538
AGGTAAC10100.013.28460112
TCTCGAG7100.013.14850356
GGTAGCG7250.013.0914177
GGAGGAT23400.013.00682116
Produced by FastQC (version 0.11.5)