FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13053632
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG896370.6866824497580444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT43000.039.5420212
GCACACG43600.038.66948711
ACGTCTG43800.038.26124615
ACACGTC44800.038.10960813
GAACTCC42500.038.05400521
CACGTCT44350.037.8653514
AGCACAC47500.036.4518410
CTCCAGT40400.035.9669824
CGTCTGA47650.035.46488616
CTGAACT48450.035.0301619
TGAACTC48900.034.06720
AACTCCA51250.033.47234722
AGAGCAC53600.032.2381448
TCCCTAC10900.032.090869
CCAGTCA35750.031.83131226
CAGTCTA5500.031.19336927
CAGTCAC32800.030.84983827
TCGGAAG58850.029.6611883
GATCGGA62300.027.24591
CGGAAGA66150.026.9163254
Produced by FastQC (version 0.11.5)