FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4798441
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT85890.178995636291037TruSeq Adapter, Index 1 (97% over 36bp)
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA74820.1559256433495796No Hit
GCGAGGTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAG51580.1074932462439363No Hit
GTTTCTTATAAGCTTTTTTCATTGAGCATCCCTCCTAAAATAGAGTACTA49930.10405462940984375No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC49260.10265834257418191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAG17150.041.63252635
TAGATCT17150.039.795839
CGATAGA18700.038.18170536
AGTCACA20050.035.7854328
TCCAGTC20600.034.66008825
ATAGATC20200.034.1335638
GTCACAG21300.033.68534529
AGATCTC20550.033.55221640
GATAGAT21650.032.33246637
TCGTATG21800.032.27054645
ACACGTC22700.032.0703913
GTATGCC22750.031.23067747
CGTATGC23450.030.74617646
CTCGTAT22800.030.54815544
CACGTCT23950.029.95815514
GCCGTCT24150.029.27527451
GAACTCC24650.029.10741821
ACTCCAG25150.028.52874223
GCACACG25900.028.10802511
AGCGATA25850.027.891634
Produced by FastQC (version 0.11.5)