FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5310068
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC112810.2124454903402367No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT94420.17781316548112No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT91220.17178687730552603No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA89880.16926336913199605No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA86860.16357605966627922No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG78370.14758756385040642No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA75770.14269120470773633No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA73110.13768185266177382No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA70570.1328984864223961No Hit
GGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCT70280.13235235405648288No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA63770.12009262404925888No Hit
GTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGA62410.11753145157463143No Hit
CGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAA62180.11709831211201062No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA58980.11107202393641662No Hit
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA57250.10781406189148614No Hit
CAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATG56470.1063451541486851No Hit
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA53330.1004318588763835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG13250.026.41519732
TCTCGCG14550.024.05507934
CTCTCGC14650.023.89087933
CACACGT15300.023.79071812
GCACACG15000.023.56653611
TCGCGCA14650.023.41306136
CTAATTA9000.023.3338643
GTGGAGA12650.023.242951
TCACTCT15300.023.10466430
ACACGTC15600.022.8844913
CGCGCAT14150.022.75627137
CAGTCAC16250.021.75372527
TCCAGTC16450.021.7020125
GTCACTC16350.021.4068129
AGCACAC17900.020.72614310
GAACTCC17250.020.08703421
CCTAGGT2453.092282E-1120.0015851
ACTCCAG17850.020.00007623
GACCACT10300.019.70862812
AGTCACT18100.019.53046428
Produced by FastQC (version 0.11.5)