FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6037105
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAAATTGTAAATTAGAATTGTCCTCCGCCCATGTTTTGTTGAGCAAAA111960.1854531269540616No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA88890.14723944672156603No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC88010.1457817944196763No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT78130.12941633448482345No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT72010.1192790252944085No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG68600.11363062262458579No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA63470.1051331722737968No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA62180.10299638651307208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGATC32100.023.66025565
ATCTCTC32150.023.6234669
GAAGCTA13600.021.61766236
GGCGTGA16900.020.09111
CCTCCGC38600.019.85769324
TGTCCTC38550.019.79249221
TCCAGTC16200.019.44461825
ACACGTC16450.019.1487913
GTGATCT40850.018.93498466
ACAAGAC41250.018.8362254
CTAATTA8200.018.7809683
GTGATAG18950.018.654834
GATTCGA32300.018.4215244
ATTGTCC41250.018.41213419
GTATGCC16050.018.31761747
TATCTCG15100.018.31112141
CCCATGT41150.018.2869230
TGATCTC42350.018.1816867
TCCGCCC41450.018.15456626
GATCTCT42800.018.15406868
Produced by FastQC (version 0.11.5)