FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089207
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA32000.1035864543878089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT7650.023.33325212
AGTCACA8300.021.08426528
GCACACG8950.020.33512511
GGTGGTG5150.019.029067
GATTCGA10800.018.4727554
ACTCCAG9700.017.68035123
AGCACAC10700.017.33638810
CCAGTCA10200.017.15680526
GGGTAAT7500.016.8013021
CAGTCAC11100.015.76571227
ACACGTC11700.015.25635713
CTCCAGT12700.014.88183824
GGATTCG14850.014.8489143
CTCCACT6900.014.71009367
CTACCTG6950.014.60426619
TCCAGTC12300.014.51214425
CTCCGAC9200.014.4576421
TGGGAGT13450.014.3133761
GTGATAG7650.014.1834163
GTGGTGA7650.014.1829588
Produced by FastQC (version 0.11.5)