FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777830
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG15350.032.83382832
CTCTCGC16300.030.70547533
CACACGT16250.030.15380112
ACTCCAG17200.029.50577223
CAGTCAC19050.027.37528627
GCACACG18300.026.96717511
CGCGCAT18350.026.89369237
GTCACTC18250.026.84927629
AGTCACT19200.026.79683528
TCACTCT18450.026.74792730
TCTCGCG18850.026.73736234
CTCGCGC19250.025.99996235
GAACTCC22100.023.28050821
TCGCGCA22100.022.48865336
TCCAGTC22400.022.34371625
AGCACAC22550.022.0398810
CTCCAGT23000.021.91301224
ACACGTC24900.020.38149513
ACGTCTG25750.019.98055315
CCAGTCA24350.019.97943726
Produced by FastQC (version 0.11.5)