Basic Statistics
Measure | Value |
---|---|
Filename | HKFJGAFXY_n01_spoVG_T1_rep1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6373495 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 10573 | 0.1658901434770091 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 8615 | 0.13516916542650462 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 8285 | 0.1299914724966443 | No Hit |
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA | 7746 | 0.12153457404453914 | No Hit |
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 7581 | 0.11894572757960899 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 7433 | 0.11662361075045953 | No Hit |
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG | 6432 | 0.10091794219654993 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACTAAT | 1960 | 0.0 | 47.321278 | 31 |
GCACACG | 2650 | 0.0 | 35.264038 | 11 |
CACACGT | 2685 | 0.0 | 35.195415 | 12 |
TGCGCAT | 2675 | 0.0 | 33.49522 | 37 |
GTCACTA | 2790 | 0.0 | 33.243618 | 29 |
ACTCCAG | 2905 | 0.0 | 32.650497 | 23 |
ATGCGCA | 2900 | 0.0 | 31.741278 | 36 |
AGTCACT | 2995 | 0.0 | 31.552488 | 28 |
ACTAATG | 2975 | 0.0 | 31.294016 | 32 |
TCCAGTC | 3075 | 0.0 | 30.503965 | 25 |
CAGTCAC | 3255 | 0.0 | 29.569796 | 27 |
ACACGTC | 3215 | 0.0 | 29.502237 | 13 |
TAATGCG | 3180 | 0.0 | 29.16657 | 34 |
GAACTCC | 3250 | 0.0 | 29.076832 | 21 |
AATGCGC | 3225 | 0.0 | 28.97665 | 35 |
AGCACAC | 3555 | 0.0 | 26.976002 | 10 |
CACGTCT | 3480 | 0.0 | 26.953936 | 14 |
ACGTCTG | 3550 | 0.0 | 26.915403 | 15 |
CTCCAGT | 3750 | 0.0 | 25.013252 | 24 |
CTAATTA | 1090 | 0.0 | 24.083057 | 3 |