FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6373495
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA105730.1658901434770091No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT86150.13516916542650462No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT82850.1299914724966443No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA77460.12153457404453914No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC75810.11894572757960899No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA74330.11662361075045953No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG64320.10091794219654993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT19600.047.32127831
GCACACG26500.035.26403811
CACACGT26850.035.19541512
TGCGCAT26750.033.4952237
GTCACTA27900.033.24361829
ACTCCAG29050.032.65049723
ATGCGCA29000.031.74127836
AGTCACT29950.031.55248828
ACTAATG29750.031.29401632
TCCAGTC30750.030.50396525
CAGTCAC32550.029.56979627
ACACGTC32150.029.50223713
TAATGCG31800.029.1665734
GAACTCC32500.029.07683221
AATGCGC32250.028.9766535
AGCACAC35550.026.97600210
CACGTCT34800.026.95393614
ACGTCTG35500.026.91540315
CTCCAGT37500.025.01325224
CTAATTA10900.024.0830573