FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7979049
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT6470368.109186946965735TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2257502.8292845425563873No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT1700972.1317954056930843No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT908921.1391332475837659No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA733770.9196208721114509No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG700190.8775356561916088No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT608690.7628603358620808TruSeq Adapter, Index 7 (97% over 35bp)
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG568210.7121274728354219No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA556970.6980405810266361No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA491560.6160633930183911No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT452900.5676115035764288No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG365860.4585258218115968No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA359350.4503669547586435No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG343430.4304147023034951No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA340510.42675511831046536No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA333070.41743069882137585TruSeq Adapter, Index 7 (97% over 35bp)
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC306170.383717407926684No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA298660.37430525868433695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTTT281070.3522600249729009TruSeq Adapter, Index 7 (97% over 35bp)
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG276210.3461690735324473No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA274450.3439632968791143No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT255110.3197248193362392No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT243020.30457263766646875No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT242460.3038707996404083No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA240910.30192821224684796No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT225260.2823143459828358No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA218480.27381709273874616No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG209450.26249995456852065No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA199920.2505561753035982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT198560.2488517115260227TruSeq Adapter, Index 7 (97% over 35bp)
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT186400.23361180010299473No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG183300.2297266253158741No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC178110.22322209075292054No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG167790.21028821855837704No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT150480.18859390385997127No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG149850.18780433608065322No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC133380.1671627784213382No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA132940.16661133425800492No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG130460.16350319442830843No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG129220.1619491245134602No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG129090.161786197828839No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT128070.16050784999565737No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT124880.1565098798114913No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGAT124020.1554320571286127TruSeq Adapter, Index 7 (97% over 35bp)
GTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTTCCGTT123940.1553317945534612No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG119520.14979228727634084No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA118930.1490528507845985No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT115200.14437810821815986No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA112810.14138276378550876No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT111790.14010441595232714No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA111150.13930231535111515No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT107870.13519154976990364No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG105130.13175755657096477No Hit
GGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCTCGTGTT100960.12653136984119284No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT100930.12649377137551104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTTT96910.12145557697414817TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95560.11976364601846662No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA94780.11878608591073948No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC94530.11847276536339105No Hit
TATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACT92400.11580327429998236No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTA91450.11461265622005831No Hit
CTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCA90230.11308365194899793No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGAT88850.11135412252763456TruSeq Adapter, Index 7 (97% over 35bp)
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACT88530.11095307222702855No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTAT88230.11057708757021044TruSeq Adapter, Index 7 (97% over 35bp)
CTATCAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTAAAAATTCT87170.10924860844945307No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT85560.10723082412452913No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA83770.10498744900551431No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG82780.1037466996380145No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT82540.10344591191256No Hit
CTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTT82470.10335818215930245No Hit
CTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTA81560.10221769536695414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT729550.064.4442244
TATGCCG754250.064.3848648
GTATGCC768350.064.2692347
TATCTCG785200.064.267441
GCCGTCT616900.064.1559751
CGTATGC745550.064.1127746
ATGCCGT712800.064.1074549
TCGTATC974900.064.0688338
CGTATCT841900.064.03818539
TGCCGTC689250.063.9975350
GTATCTC796000.063.99342340
CCGTCTT639650.063.8987152
ATTCGTA1010450.063.88302236
TTCGTAT1000600.063.8088237
AATTCGT1014100.063.76699435
GAATTCG1017200.063.67931734
TCTCGTA740850.063.66441343
ATCTCGT775300.063.63442642
CGAATTC1018600.063.57461533
TCGTATG716900.063.56505245
Produced by FastQC (version 0.11.5)