FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spoVG_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spoVG_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284881
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTCG13450.030.18579732
CACACGT15800.026.58221212
CTCTCGC15250.026.16386833
GCACACG16300.025.5520811
ACTCCAG16450.025.53184923
ACTCTAG6850.021.9709555
TCTCGCG18050.021.71739634
AGTCACT18950.021.42474728
AGCACAC20700.020.62796610
CAGTCAC20650.020.50842327
GTCACTC19300.020.3108329
TCACTCT19500.020.10251230
GAACTCC19750.020.02545521
CGCGCAT19300.019.40409537
CTCGCGC20400.019.38720535
CCAGTCA21900.018.69858226
CGACCCA3001.8189894E-1218.6667964
CTCCAGT21700.018.38704924
TCCAGTC22800.018.11398925
CTATAGG1953.9564839E-717.94867111
Produced by FastQC (version 0.11.5)