HKFJGAFXY_n01_spoVG_T0_rep1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKFJGAFXY_n01_spoVG_T0_rep1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4033194
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	10097	0.25034749134308937	TruSeq Adapter, Index 7 (97% over 36bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA	7979	0.19783328052159158	No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT	6186	0.15337719931151342	No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA	5436	0.1347815155928527	No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC	5422	0.13443439616343772	No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT	5240	0.12992184358104272	No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA	4811	0.1192851124939688	No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG	4784	0.11861566788009702	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGTCACC	1990	40.62804	28
ACTCCAG	2005	40.498653	23
GCACACG	2115	38.39234	11
CAGTCAC	2225	37.280807	27
CACACGT	2195	36.993076	12
TCCAGTC	2280	36.22798	25
GTATGCC	2185	36.201283	47
CCGGCTA	2240	35.937412	33
GCTATGA	2325	35.526794	36
GAACTCC	2350	34.70204	21
CTCCAGT	2350	34.70204	24
TCGTATG	2320	34.54733	45
CTATGAT	2395	33.46547	37
GGCTATG	2465	33.367058	35
AGCACAC	2515	32.2862	10
CGTATGC	2485	32.25344	46
ACGTCTG	2545	32.04314	15
ACACGTC	2575	31.669823	13
CGGCTAT	2665	30.862963	34
CACGTCT	2695	30.649273	14