Basic Statistics
Measure | Value |
---|---|
Filename | HKFJGAFXY_n01_spo0A_T2_rep3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2332840 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC | 3994 | 0.17120762675537113 | No Hit |
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA | 3350 | 0.14360179009276247 | No Hit |
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT | 2845 | 0.12195435606385349 | No Hit |
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT | 2784 | 0.1193395174979853 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA | 2629 | 0.11269525556832016 | No Hit |
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG | 2421 | 0.10377908472076953 | No Hit |
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA | 2402 | 0.10296462680681058 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCCAGT | 1370 | 0.0 | 44.19677 | 24 |
GAACTCC | 1420 | 0.0 | 42.640545 | 21 |
ACTCTCG | 1405 | 0.0 | 42.099346 | 32 |
CTCTCGC | 1430 | 0.0 | 41.6081 | 33 |
TCTCGCG | 1440 | 0.0 | 41.076096 | 34 |
TCGCGCA | 1475 | 0.0 | 41.05056 | 36 |
TCCAGTC | 1505 | 0.0 | 40.232277 | 25 |
CTCGCGC | 1480 | 0.0 | 40.202423 | 35 |
CACGTCT | 1575 | 0.0 | 38.666397 | 14 |
CGCGCAT | 1495 | 0.0 | 38.62849 | 37 |
TGAACTC | 1575 | 0.0 | 38.221954 | 20 |
ACTCCAG | 1600 | 0.0 | 38.062233 | 23 |
TCACTCT | 1580 | 0.0 | 37.436443 | 30 |
ACACGTC | 1630 | 0.0 | 37.14698 | 13 |
CACACGT | 1635 | 0.0 | 36.819313 | 12 |
ACGTCTG | 1660 | 0.0 | 36.264805 | 15 |
CCAGTCA | 1660 | 0.0 | 36.264805 | 26 |
GCACACG | 1710 | 0.0 | 34.999752 | 11 |
AACTCCA | 1800 | 0.0 | 34.22198 | 22 |
GCGCATC | 1730 | 0.0 | 32.976646 | 38 |