FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T2_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T2_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332840
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC39940.17120762675537113No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA33500.14360179009276247No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT28450.12195435606385349No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT27840.1193395174979853No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA26290.11269525556832016No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG24210.10377908472076953No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA24020.10296462680681058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGT13700.044.1967724
GAACTCC14200.042.64054521
ACTCTCG14050.042.09934632
CTCTCGC14300.041.608133
TCTCGCG14400.041.07609634
TCGCGCA14750.041.0505636
TCCAGTC15050.040.23227725
CTCGCGC14800.040.20242335
CACGTCT15750.038.66639714
CGCGCAT14950.038.6284937
TGAACTC15750.038.22195420
ACTCCAG16000.038.06223323
TCACTCT15800.037.43644330
ACACGTC16300.037.1469813
CACACGT16350.036.81931312
ACGTCTG16600.036.26480515
CCAGTCA16600.036.26480526
GCACACG17100.034.99975211
AACTCCA18000.034.2219822
GCGCATC17300.032.97664638