FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T2_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T2_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943715
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA46770.11859376248030094No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT41090.10419109900182949No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC39920.10122435317967958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT12900.048.0235631
GTCACTA14400.043.26416429
ACTAATG15150.040.6598132
GAACTCC17050.036.5398221
TGCGCAT16450.036.16998337
TCACTAA19200.032.81270630
ATGCGCA19400.032.2936136
CACACGT20000.031.84979812
TCCAGTC19650.031.70503625
GCACACG19900.031.48221211
CTCCAGT20700.030.43497524
ACTCCAG20650.030.00018923
CACGTCT21900.029.08657314
ACGTCTG22900.027.81641615
AATGCGC22350.027.40474935
ACACGTC23750.027.26298313
AGTCACT24000.026.54183428
ACTCTAG8250.025.8796085
AGCACAC24850.025.77448510
GCGCATC22750.025.69214438
Produced by FastQC (version 0.11.5)