FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T2_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T2_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556072
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC71030.1278421157969155No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT70810.12744615260565376No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA64890.11679114309533786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG34950.046.4666333
ACTCCGC36150.044.9241732
GAACTCC36700.044.34628721
TCCAGTC38850.041.9822125
CACGTCT39550.041.59277314
CACACGT42000.038.9998612
ACGTCTG41850.038.9723815
ACTCCAG42350.038.2646723
GCACACG42900.038.01851311
TCACTCC43900.037.39200230
GTCACTC43850.037.0355529
ACACGTC45000.036.86653513
AGTCACT45350.036.4279828
CCGCGAA45200.036.23913235
CTCCAGT44850.036.13174424
AACTCCA48350.033.87815522
CGCGAAA50300.032.0777136
TCCGCGA51600.031.9478534
CACTCCG52850.031.85447931
CTCTAGT16900.030.650786
Produced by FastQC (version 0.11.5)