FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T1_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T1_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787673
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT54060.1939251841948464TruSeq Adapter, Index 1 (97% over 36bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA49580.1778544327114407No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT39610.14208983621823648No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC36430.13068247244206907No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA33920.12167854694578598No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG32390.11619009833649786No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT30050.107796000463469No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA29890.10722204505334736No Hit
CGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCAAA29320.10517732890478904No Hit
GCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAATATA28500.1022358074279157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT10900.034.0366639
ATAGATC12850.029.41631338
AGATCTC12600.029.1666440
GCGATAG13450.028.62450635
GCACACG13750.028.50906211
GAACTCC13850.028.3032221
GTATGCC13850.027.545147
CACACGT14050.027.40210712
CTCCAGT14650.026.51874424
CGATAGA15200.026.0197136
AGTCACA15350.025.99345828
CTCGTAT14700.025.71425844
GTCACAG15500.025.51610229
ACTCTAG8000.025.3749755
CTCTAGT8000.025.3749756
GATAGAT15350.025.3094237
TCCAGTC15550.025.20897925
ACTCCAG15600.025.12817823
CACGTCT15800.024.58858314
CCAGTCA15850.024.29019726
Produced by FastQC (version 0.11.5)