FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T1_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T1_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4530616
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA56450.12459674357747379No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT47470.10477603928472419TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC12400.033.0243721
CTATGAT14100.028.04948237
GTATGCC14950.027.39114647
AGTCACC15500.027.32272328
CCGGCTA15050.026.97658733
ACGTCTG15100.026.88725915
TCGTATG15100.025.96011445
ACTCCAG15850.025.83609823
TCCAGTC16200.025.71000925
CTCTAGT6050.025.455246
CACGTCT16100.025.21724314
CTCGTAT15400.024.99985344
GTACTCT6250.024.6406733
CACACGT17750.023.46465112
GCTATGA17700.023.33319736
ACACGTC18150.023.1403613
GGCTATG18600.022.95685635
CGGCTAT18050.022.88075334
GCACACG18350.022.69741411
CGTATGC18000.022.36098146
Produced by FastQC (version 0.11.5)