FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T1_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T1_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3896452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA71400.1832436277926688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT56560.145157697310271TruSeq Adapter, Index 19 (97% over 38bp)
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT54880.14084608253867878No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC53320.13684244025077172No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA50150.1287068338067555No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA47920.12298367848493964No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT47480.12185444604476071No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG46570.11951898804348161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCTA13500.033.9631736
TATCTCG14250.030.70154841
CTCGTAT14150.030.67117144
GAACTCC15350.030.5539321
GTATGCC14900.029.83201447
TCGTATG15050.029.30212645
TCCAGTC15200.029.01333425
AGTCACC16050.027.25873228
CACGTCT17150.027.14267314
CCAATAG5400.026.57389538
ACGTCTG17550.026.12517715
GTCACCT16650.026.06622129
GCCGTCT17200.025.63935951
GCACACG18150.025.45437211
ACTCCAG17550.025.32778723
CGTATGC18550.024.71681246
CACACGT18650.024.58428412
ACTAGAG6250.023.52014420
CTAGAGT6800.023.16190521
ACACGTC20900.022.60750213
Produced by FastQC (version 0.11.5)