FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T0_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T0_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447267
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT28090.19408996404948084TruSeq Adapter, Index 13 (97% over 38bp)
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA20700.14302820419452664No Hit
TGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAG19340.1336311820831954No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT19090.1319037883127301No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC16840.11635724437854245No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA16770.11587357412281218No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT16760.11580447837199355No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA16680.11525171236544465No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA15040.10392000923119232No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA14840.10253809421482007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCC400.002419680134.9998854
GAACTCC8200.032.01208521
AGTCACA8900.029.88753928
CTCCAGT8550.029.88293624
CACATTC9350.028.44909931
CTCTAGT3500.028.0008726
CACGTCT9850.027.7156414
TCCAGTC9750.026.5640125
ACTCCAG9950.026.38181923
CACACGT10450.026.12431312
GCACACG10300.025.82515311
GTCACAT10250.025.60966929
CTAACGT3350.025.0762731
AGCACAC10900.025.04578610
TGAACTC10350.025.02406920
AACTCCA10600.024.76406522
CCAGTCA10950.024.61178826
ACGTCTG10850.024.51604315
ACTCTAG4500.024.111865
ACACGTC11200.023.74991813
Produced by FastQC (version 0.11.5)