FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T0_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T0_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5070954
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT105910.20885616394863768TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC19850.042.14097221
AGTCACC21800.037.72928628
CTATGAT22100.036.7420137
CCGGCTA23000.036.67383633
ACTCCAG22750.036.46146823
CTCGTAT22700.035.46248244
GCACACG24400.034.56960711
GCTATGA24600.034.2885536
TCCAGTC24750.034.22215325
CTCCAGT24450.033.9263124
ACGTCTG24950.033.38670715
TCGTATG25300.032.78649545
AGCACAC26550.032.42931410
CACACGT26850.031.54556312
GTATGCC27300.031.41019647
AACTCCA27200.031.13964722
CACGTCT27200.030.75361814
GGCTATG28050.029.69691335
CGTATGC29450.028.52286546
GCCGTCT29100.028.38482351
Produced by FastQC (version 0.11.5)