FastQCFastQC Report
Fri 14 Jun 2019
HKFJGAFXY_n01_spo0A_T0_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFJGAFXY_n01_spo0A_T0_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261949
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGA56680.1737611470933482No Hit
GTGGAGACGGTGGGAGTCGAACCCACGTCCAGAAACATCGATTACTTAAG54430.16686343042150567No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC53260.16327661775214758No Hit
TCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAA50000.15328259270761133No Hit
CCCACGTCCAGAAACATCGATTACTTAAGCGTCTACGAGCGTAGCCTGCA47160.144576141441819No Hit
GATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCA40790.1250479391308693No Hit
CGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACACGCACTTAAAT40520.12422021313024821No Hit
CTCGTAAACACGCACTTAAATATAACTGGCAAAACTAACAGTTTTAACCA40090.12290198283296276No Hit
CAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGATCTCGTAAACA38010.1165254269763261No Hit
ACGGATTCGACAGGGATGGATCGAGCTTGAGCTGCGAGCCGAGAGGCGAT35960.11024084067531405No Hit
CGATTACTTAAGCGTCTACGAGCGTAGCCTGCATATTTATGGTTTCACTC35330.10830948000719814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAT22400.051.719231
GTCACTA23050.050.108929
CTAGAGT10800.047.63857721
ACTAATG24400.047.33648332
TGCGCAT24300.046.5230437
CCAATAG11100.045.7204238
ATGCGCA25800.044.49651336
GAACTCC26600.043.4207721
TCACTAA26900.042.9371830
AATGCGC27700.041.94982535
CTCCAGT27900.041.27276624
ACTAGAG12650.040.6716720
CAATAGA12950.039.7294739
TCCAGTC29200.039.5551425
ACTCCAG29500.039.27092723
ACGTCTG29600.038.66528715
AGTCACT30250.038.52926328
AACTAGA13550.038.2285319
AGAGTAC13700.037.55449723
GCACACG31450.036.94729211